NERIC 2017 Speakers

Vicki P. Losick, PhD

Vicki P. Losick, PhD

Education

Postdoc with Allan Spradling at Carnegie Institution
Ph.D. Tufts Medical School
B.S. Bates College

Research Interests

Scientists have been working with the fruit fly, Drosophila melanogaster, for more than 100 years to answer fundamental questions in biology. The Losick Lab takes advantage of the ease and power of Drosophila genetics to understand the basic mechanisms of tissue repair.

Wound healing is not a one-size-fits-all process. While the typical wound healing response relies on cell division to regenerate the cells that are lost by injury, aging, or disease. We found that cells can also be replaced by stimulating existing cells to grow in size by becoming polyploid, a process named wound-induced polyploidization (WIP). A polyploid cell is a cell that has more than diploid copy of its chromosomes. As a result, polyploid cells can grow to be many orders of magnitude larger than their diploid cell counterparts. Polyploid cells are ubiquitous in insects and plants, but also are also required for the development of many tissues in our body. What remains unknown is why under conditions of stress, like injury, aging, and disease, polyploid cells frequently arise? and how do polyploid cells function during these stress responses?

We are beginning to answer these fundamental questions using the fruit fly as a model. Fruit fly puncture wounds (Figure 1) heal by WIP where cells grow in size forming both mono and multi-nucleated enlarged cells (Figure 2). We found that cell enlargement through polyploidization is required for wound healing, since wounds fail to close if WIP is genetically perturbed in Drosophila. In collaborative work, we recently discovered that WIP also functions in mammals to maintain the size and functional capacity of the repaired tissue by enlarging existing cells in mouse cornea endothelium. Therefore, WIP appears to be a conserved and widely used part of the body’s healing arsenal. The Losick laboratory is currently studying the molecular mechanisms regulating WIP in flies and mice in order to understand how to generate these extra-large cells to improve our bodies ability to heal.

Selected Publications

Losick, VP, Jun, AS, and Spradling, AC. Wound-Induced Polyploidization: Regulation by Hippo and JNK Signaling and Conservation in Mammals. PLoS One. 2016 Mar 9;11(3):e0151251.

Losick VP. Wound-induced polyploidy is required for tissue repair. Adv Wound Care. 2014, ahead of print. doi:10.1089/wound.2014.0545. Review.

Losick, VP, Fox DT, and Spradling, AC. Polyploidization and cell fusion contribute to wound healing in the adult Drosophila epithelium. Current Biology. 2013, Nov 18; 23(22):2224-32.

Liu M, Uehara T, Losick VP, Park JT, and Isberg RR. A Legionella pneumophila protein that facilitates evasion from Nod1 recognition. Cell Host Microbe. 2012, Aug 16; 12(2):166-76.

Losick VP, Morris LX, Fox DT, Spradling A. Drosophila stem cell niches: a decade of discovery suggest a unified view of stem cell regulation. Developmental Cell. 2011, Jul 19; 21(1):159-71. Review.

Losick VP, Haenssler E, Moy M, and Isberg RR. LnaB: a Legionella pneumophila activator of NF-kappaB. Cell Microbiology. 2010, Aug; 12(8):1083-97.

Li Z, Dugan AS, Bloomfield G, Skelton J, Ivens A, Losick VP, and Isberg RR. The amoebal MAP kinase response to Legionella pneumophila is regulated by DupA. Cell Host Microbe. 2009, Sep 17; 6(3):253-67.

Losick VP*, Stephan KS*, Isberg, RR, Poltorak A. A hemidominant Naip5 allele in MOLF/Ei-derived macrophages restricts L. pneumophila intracellular growth. Infection and Immunity. 2009, Jan; 77(1):196-204. *Co-primary authors.

Losick VP and Isberg RR. NF-appaB translocation prevents host cell death after low-dose challenge by Legionella pneumophila. Journal of Experimental Medicine. 2006, Sep 4; 203(9):2177-89.

Losick VP, Schlax PE, Emmons RA, Lawson TG. Signals in Hepatitis A Virus P3 region proteins recognized by the ubiquitin-mediated proteolytic system. Virology. 2003, May 10; 309(2):306-19.

Agenda

(Information subject to change)

Wednesday, August 16th

2:00 - 9:00 PM Registration Desk Hours of Operation (Diamond Foyer)
5:00 - 6:00 PM Reception and Poster Session I Setup (Promenade)
6:00 - 6:15 PM Welcoming Remarks (Emerald I-III Ballrooms)
6:15 - 7:30 PM Plated Dinner (Emerald I-III Ballrooms)
7:30 - 9:00 PM Poster Session I (Undergraduate students and Cores) (Promenade)

Thursday, August 17th

7:00 AM - 5:30 PM Registration Desk Hours of Operation (Diamond Foyer)
7:00 - 8:15 AM Breakfast, Career Tables for interested students (Exhibition Hall), Poster Session II Set-up (Promenade)
  • Tim Hunter, MS, Biotek and Yolanda Nesbeth PhD, Celdara Medical
    "Biotech Career"
  • Corine Farewell, DVM, MBA UVM Innovations
    "Technology Transfer"
  • Charles Irvin, PhD, UVM College of Medicine, Director, Vermont Lung Center, and Ralph Budd, MD, UVM College of Medicine, Director, Vermont Center for Immunology & Infectious Diseases
    "Academic Career"
  • Yanping Liu, MD, PhD, NIH, NIGMS
    "Scientific Careers in Government"
  • Daniel Kolker, PhD, United States Patent and Trademark Office
    “Patent Assessment”
  • Karen Spach, PhD, Senior Medical Writer, Envision Pharma Group
    "Scientific Careers in Government"
8:30 - 9:45 AM Opening Remarks (Emerald I-III Ballrooms) NIGMS staff:
  • Yanping Liu, MD, PhD Program Director, NIGMS
    “Overview of Programs in the Center for Research Capacity Building”
  • Arina Kramer, Grants Specialist at the Center for Research Capacity Building, NIGMS
    “IDeA Post Award Management”
9:45 - 10:00 AM Break and Refreshments (Promenade)
10:00 - 12:00 PM Concurrent Sessions
  1. Core Presentations of Resources Available to NERIC Investigators
    (Emerald III Ballroom) Chair, Ralph Budd, MD, University Distinguished Professor of Medicine, The University of Vermont Larner College of Medicine
    • Eric Kmiec, PhD, Helen F. Graham Cancer Center & Research Institute, Newark, DE
      "CRISPR-based Gene Editing in Biomedical Research"
    • Youngwook Ahn, PhD, Brown University Transgenics and Gene-Targeting Facility
      “CRISPR/Cas9 Mouse Preparation”
    • Bongsup Cho, PhD, Coordinator INBRE and Professor, The University of Rhode Island
      "Expanding Bioinformatic Core: 3D Printing of Molecules and Viruses!”
    • Nagib Ahsan, PhD, Brown University
      "Proteomics Core Facility"
    • Calvin Vary, PhD, Maine Medical Research Institute
      "Lipidomics"
    • Dwight Matthews, PhD, University of Vermont
      "Metabolomics and 13C-labeling"
  2. Entrepreneurship Workshop: "How to Turn Your Innovative Ideas Into Reality" (Emerald II Ballroom)
    • Mercedes Rincón, PhD,   University of Vermont
      "Welcome to the I-Trep program for IDeA states"
    • Keynote speaker, Mathew Portnoy, PhD, Overall HHS SBIR/STTR Program Manager, NIH Program Manager, NIH Office of Extramural Programs
    • Pitch presentations
      • Voot Yin, PhD, MDI Biological Laboratory
      • Qian Chen, PhD, The Warren Alpert Medical School of Brown University/Rhode Island Hospital
    • Round tables (20 minutes each, choose three)
      • Daniel Kolker, PhD, Supervisory Patent Officer, USPTO, and MaryDilys Anderson, PhD, Esq, Finch & Maloney PLLC)
        “Assessing your Invention and Intellectual Property”
      • Samantha Roach-Gerber, VCET, and Erik Monsen, PhD, University of Vermont
        “How To Pitch-How to Sell Your Idea”
      • Cairn Cross, Fresh Tracks Venture
        “Funding Through VC, Angels”
      • Mathew Portnoy, PhD, NIH SBIR Program, and Yolanda Nesbeth, PhD, Celdara Medical
        “Funding Through SBIR/STTR”
      • Sarah O'Connell Kalil, COO, Boston Microfluidics, and Tanya Kanigan, PhD, COO, Genomic Expression Inc.
        “Setting Up Your Startup”
      • Tina Thornton, PhD, University of Vermont
        “I-Trep Program Opportunities”
  3. INBRE Program (Emerald I Ballroom)
    • 10:00 - 11:00 AM - Sharon O'Connor, MS, MBA, Internal Evaluation Manager, Dartmouth College, New Hampshire INBRE
      "INBRE Program Evaluation"
    • 11:00 - 12:00 PM - Elizabeth Ambos, PhD, Executive Officer, Council on Undergraduate Research
      "PM Council on Undergraduate Research Presentation"
10:30 - 11:30 AM COBRE/INBRE Business Grant Managers Meeting with Arina Kramer, Grants Specialist at the Center for Research Capacity Building, NIGMS (Carlton Boardroom)
12:00 - 1:30 PM U.S. Senator Patrick Leahy (Exhibition Hall)

Lunch and Networking (Exhibition Hall)
VGN Baccalaureate Partner Institution Meeting (Carlton Boardroom)
1:30 - 3:30 PM Concurrent Scientific Sessions
Development/Genetics (Emerald I Ballroom) Chair, Bruce Stanton, PhD, Professor of Microbiology and Immunology, Geisel School of Medicine, Dartmouth
  • Featured Speaker: Vicki Losick, PhD, MDI Biological Laboratory
    “Wound Healing the Fruit Fly Way”
  • Featured Speaker: Jodi Camberg, PhD, University of Rhode Island
    "Remodeling protein architectures leads to big changes in cell physiology"
  • Santina Snow, MDI Biological Laboratory
    “The Role of Specific Tissues in Coordinating Changes in Organismal Growth, Longevity and Resilience to Stress in Response to Reduced mRNA Translation in C. Elegans”
  • Aaron Brown, PhD, Maine Medical Center Research Institute
    “A Novel Human Pluripotent Stem Cell-Derived Model of Beige Adipogenesis for Development of Anti-Obesity Therapies”
  • Larissa Williams, PhD, Bates College
    “The Transcription Factor, Nuclear Factor, Erythroid 2 (Nfe2), is a Regulator of the Oxidative Stress Response During Danio Rerio Development”
  • Michaela Reagan, PhD, Tufts University
    “3D Tissue Engineered Design of Bone Marrow Adipose Tissue to Investigate the Influence of Myeloma on Adipocytes and Adipogenesis
Cancer (Emerald III Ballroom) Chair, Mercedes Rincon, PhD, Professor of Medicine, The University of Vermont Larner College of Medicine
  • Featured Speaker: Patrycja Dubielecka, PhD, Brown University
    “Beyond JAK/STAT: Signaling Pathways Contributing to the Pathogenesis of Myeloproliferative Neoplasms”
  • Sonali Barwe, PhD, Alfred I. DuPont Hospital for Children
    “Disseminated Patient-Derived Xenograft Models for Preclinical Testing of New Drugs for Pediatric Leukemia”
  • Mark Perfetto, University of Delaware
    “ADAM9 Cleaves EphrinB1 to Regulate Canonical Wnt Signaling and Colorectal Cancer Cell Migration”
  • Con Sullivan, PhD, University of Maine
    "A Role for the Tumor Necrosis Factor-Alpha-Induced Protein 8-like 1 Gene in Cancer and Inflammation"
  • Sigrid Langhans, PhD, Nemours/Alfred I DuPont Hospital for Children
    “Beta-Hairpin Peptide Hydrogels as Scaffolds for 3D High-Throughput Screening in Drug Discovery”
  • Andrea Villanti, PhD, MPH, Associate Professor, Vermont Center on Behavior and Health, Department of Psychiatry, University of Vermont
    “Characteristics of Young Adult Cigarette Smokers in the U.S.: Findings from the 1st Wave of the PATH Study, 2013-2014”
Infectious Diseases and Immunology (Emerald II Ballroom) Chair, William Green, PhD, Professor of Microbiology and Immunology, Geisel School of Medicine, Dartmouth
  • Featured Speaker: William Landesman, PhD, Green Mountain College
    “Drivers of Variation in theIxodes Scapularis Microbiome”
  • Jason Shapiro, MD, Alpert Medical School, Brown University
    “Immunoglobulin-A Coated Bacteria Represent a Unique Subset of the Microbiome in Patients with Inflammatory Bowel Disease”
  • Md Mahmudul Hasan, University of Vermont
    “Motility of E. Histolytica Through Host Extracellular Matrix: Involvement of Invadosome-like Actin Structures”
  • Rajiv Jumani, University of Vermont
    “Identification and Pharmacokinetic-Pharmacodynamic Modeling of a Piperazine-Based Drug Lead for Cryptosporidiosis”
  • Hacene Boukari, PhD, Delaware State University
    “Tracking HIV Viruses in Human Cervical Mucus”
  • Victoria DeVault, University of Vermont
    “Natural Genetic Variation within a 0.1Mbp Genetic Interval on Chromosome 1 Regulates NKT Cell Development and Function”
3:30 - 3:45 PM Break and Refreshments (Promenade)
3:45 - 4:45 PM Keynote Lecture I (Emerald III Ballroom)
  • Jason McLellan, PhD, Geisel School of Medicine at Dartmouth
    “Developing New Therapeutic Interventions to RSV Infection by Inhibiting the Metamorphosis of the RSV Fusion Glycoprotein”
4:45 - 6:00 PM Poster Session II (Graduate students and Postdoctoral Fellows) (Promenade)
5:30 - 6:30 PM NIH COBRE and INBRE PI Meeting
(Universal Amphitheater)
6:00 PM Dinner on your own in Burlington

Friday, August 18th

8:00 - 9:00 AM Breakfast (Exhibition Hall)
9:00 - 10:00 AM Cool ideas from IDeA programs (Emerald III Ballroom) Chair, Charles Irvin, PhD, Professor of Medicine, The University of Vermont Larner College of Medicine
  • Bruce Stanton, PhD, Professor of Microbiology and Immunology, Geisel School of Medicine, Dartmouth
    “Leveraging COBRE Resources with Philanthropy”
  • Marie Stuart, MD, PI of SCD COBRE Program, Director of Hematology Research A.I. DuPont Hospital for Children
    “Single Disease Centered COBREs…A Novel Approach”
  • W. Kelley Thomas, PhD, Professor, Dept. Molecular, Cellular and Biomedical Sciences, University of New Hampshire
    “Early Undergraduate Experiences in Bioinformatics”
10:00 - 12:00 PM Concurrent Scientific Sessions Neuroscience (Emerald I Ballroom) Chair, Melissa A. Harrington, PhD, Associate VP for Research, Delaware State University
  • Matthew E. R. Butchbach, PhD, Nemours/Alfred I. duPont Hospital for Children
    "Characterization of Small Molecule Neuroprotectants for Spinal Muscular Atrophy”
  • Featured Speaker: Andrew Kennedy, PhD, Bates College
    "Targeting the Tet Enzymes to Enhance Cognition"
  • Neal Mecum, University of New England
    “Lacrimal Gland Excision in Male and Female C57BL/6 Mice Causes Pain- and Anxiety-like Behavior”
  • Michael Dash, PhD, Middlebury College
    “Synaptic Depotentiation Reduces Sleep Need”
  • Leslie Johnson, PhD, Johnson State College
    “More than Just ‘Baby Weight’: Perceived Postpartum Weight Bias and Well-Being in First-Time Mothers”

Infectious Diseases and Immunology II (Emerald III Ballroom) Chair, Ralph Budd, MD, University Distinguished Professor, Medicine, University of Vermont Larner College of Medicine
  • Michael Secinaro, University of Vermont
    “Glycolysis Primes Effector T Cells for Death Through the Induction of Caspase-3 Activity”
  • Melissa Maginnis, PhD, University of Maine
    “Characterization of Cellular Factors Required for JC Polyomavirus Invasion of Host Cells”
  • Abbas Raza, University of Vermont
    “Sex-Specific Regulation of CNS Autoimmunity by the Signal Lymphocytic Activation Molecule (Slam) Locus”
  • Danielle Dube, PhD, Bowdoin College
    “Discovery of Genes Required for Glycoprotein Biosynthesis in the Gastric Pathogen Helicobacter Pylori”
  • Scott Rusin, Dartmouth College
    “Identification of Candidate CK2 Substrates in Mitosis by Quantitative Phosphoproteomics”
Bioinformatics, Computational Biology, Complex Systems (Emerald II Ballroom) Chair, Cathy Wu, PhD, Edward G. Jefferson Chair of Bioinformatics & Computational Biology, University of Delaware
  • Featured Speaker: Benjamin King, PhD, University of Maine
    "microRNA co-expression network dynamics of Pseudomonas aeruginosa infection in the zebrafish, Danio rerio"
  • Featured Speakers: Dr. Malcolm D’Souza and Dr. Derald Wentzien from Delaware INBRE Wesley College
    “Undergraduate Data Science Research Projects Form an Integral Component of the Wesley College Science and Mathematics Curriculum”
  • Shawn Polson, PhD, University of Delaware
    “Markers to Metagenomes and Back Again: Bioinformatics advances toward understanding microbial viruses”
  • Edward Lyman, PhD, University of Delaware
    “Computational Approaches to GPCR Drug Discovery: The Membrane as a Therapeutic Target”
  • Cathy Wu, PhD, University of Delaware
    “Big Data to Knowledge: Integrative Literature Mining and Panomics Analysis for Kinase Cancer Drug Analytics”
12:00 - 1:00 PM Lunch and Networking Box lunches (G's Restaurant)
1:00 PM Conference Concludes